MutMap Approach Enables Rapid Identification of Candidate Genes and Development of Markers Associated With Early Flowering and Enhanced Seed Size in Chickpea (Cicer arietinum L.)

Globally terminal drought is one of the major constraints to chickpea ( Cicer arietinum L.) production. Early flowering genotypes escape drought, and increase in seed size compensates for yield losses arising from drought. A MutMap population early large was developed by crossing mutant line ICC4958-M3-2828 with wild-type ICC 4958. Based on phenotyping population, extreme bulks days 100-seed weight were sequenced using Hi-Seq2500 at 10X coverage. On aligning 47.41 million filtered reads CDC Frontier reference genome, 31.41 mapped 332,395 single nucleotide polymorphisms (SNPs) called. genome assembly 4958 replacing these SNPs particular positions genome. specific each bulk ranged 3,993 5,771. We report a unique genomic region Ca6 (between 9.76 12.96 Mb) harboring 31, 22, 17, 32 peak SNP index = 1 low time, high weight, respectively. Among these, 22 are present 20 candidate genes had moderate allelic impact genes. Two markers, Ca6EF10509893 Ca6HSDW10099486 validated SNPs. Thus, associated genes, SNPs, markers this study useful breeding varieties that mitigate under stress.